980-nm diyot lazer ile oluşturulan deneysel spinal kord travması modelinde n-asetil sistein ve vitamin E’nin nöroprotektif etkisi








































































ABSTRACT
In this work, the neuroprotective effect of N- acetyl cisteine (NAS) and Vitamin E (E) on experimental spinal cord injury caused by experimental 980- nm diode laser had been investigated. There were 6 rat groups, each of these groups consists of 6 wistar albino rats. No medical treatment and trauma had been applied to these rats in the control group, where as only laminectomy had been applied to the rats in the laminectomy group. After laminectomy the rats in the laser group had been applied to 980- nm diode laser (10 joule) for 2 seconds to cause spinal cord injury. One hour later after the laser trauma, 100mg/kg intraperitoneal NAS was given to NAS group and 30mg/kg intraperitoneal E was given to the rats in the E group. The rats in the E + NAS group were applied to the above doses E + NAS. The animals were sacrificed at the posttraumatic 5th day. Neurological, biochemical (malondialdehide (MDA), total antioxidant status (TAS)), histopathological evaluations were done. Based on the histopatological evaluation, no significant difference among each groups except the control group was noticed. In evoluation of neurological examination results, the treatment groups treated with E and NAS separately, it was observed that the neurologic examination performances at the 5th day has significantly rised according to trauma group. No significant difference was noticied by using the combination treatment. The results reveal that when applying E and NAS separately, the clinic neurological examination scores significantly had increased. It was pointed that functional and clinical improvements had been occured. In evaluation of biochemical results; when the control group and laminectomy group compared difference at the MDA (nmol/gr-tissue) values determined sicnificant statically ( p:0.016). We thought that, this difference have been occurs because of aedema after laminectomy. This aedema had been verified pathologically. When the control group is compared with the laser and the treatment group, no significant difference had been noticed. When the laser group was compared with the treatment groups, no significant difference had been noticed. These biochemical results reveal that E and NAS s treatment dose, applying time and rats sacrification time had been tought to be corallated. As a conclusion, the application of E and NAS separately in spinal cord injury clinical neurologic treatment had been observed. These treatments may tought to be used in the future after clinical investigations.
Key Words : 980- nm diode laser, spinal cord injury, Malondialdehide, Total antioxidant status, Nacetyl cisteine, Vitamin E.
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5. SAYFAYA BENZER SAYFALAR

Deneysel omurilik yaralanmasında Etomidat'ın nöroprotektif etkinliğinin değerlendirilmesi - Sayfa 11
EVALUATION OF NEUROPROTECTIVE EFFICACY OF ETOMIDATE IN EXPERIMENTAL SPINAL CORD INJURY (SCI) Cenk Ergüden, MD., Dokuz Eylul University, School of Medicine, Department of Neurosurgery, Inciralti – Izmir / Turkey ABSTRACT Objective: Purpose of this study was to evaluate the neuroprotective efficacy of preand post- traumatic Etomidate administration in histopathological and neurobehavioral recovery...
Deneysel spinal kord travmasında alfa-lipoik asidin nöroprotektif etkilerinin metilprednizolone ile karşılaştırmalı incelenmesi - Sayfa 6
COMPARISON OF NEUROPROTECTIVE EFFECTS ON ALPHA LIPOIC ACID TO METHYLPREDNIZONOL AT EXPERIMENTAL SPINAL CORD TRAUMA Traumatic and ischemic injuries of spinal cord are active at malfunctionıng of damaged tissue at primary and secondary mechanisms. Monoammins, free radicales, neuropeptits, arachidonic acids metobolites, and extracellular Ca variations are important at development of early ischemic a...
Deneysel akut spinal kord hasarında quetıapıne' in tedavideki etkisi - Sayfa 99
89 8. SUMMARY The importance of treatment modalities against damage on spinal cord at seconder phase of spinal cord injury is well known. So, numerous experimental studies have been focused on this phase. Effectiveness of Quetiapine (Serequel©), a 5-HT2 receptor blocker atypical antipsychotic agent, on apoptosis that is one of the major mechanisms of secondary damage on spinal cord was research...

5. SAYFADAKI ANAHTAR KELIMELER

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group
trauma
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injury


5. SAYFA ICERIGI

ABSTRACT
In this work, the neuroprotective effect of N- acetyl cisteine (NAS) and Vitamin E (E) on experimental spinal cord injury caused by experimental 980- nm diode laser had been investigated. There were 6 rat groups, each of these groups consists of 6 wistar albino rats. No medical treatment and trauma had been applied to these rats in the control group, where as only laminectomy had been applied to the rats in the laminectomy group. After laminectomy the rats in the laser group had been applied to 980- nm diode laser (10 joule) for 2 seconds to cause spinal cord injury. One hour later after the laser trauma, 100mg/kg intraperitoneal NAS was given to NAS group and 30mg/kg intraperitoneal E was given to the rats in the E group. The rats in the E + NAS group were applied to the above doses E + NAS. The animals were sacrificed at the posttraumatic 5th day. Neurological, biochemical (malondialdehide (MDA), total antioxidant status (TAS)), histopathological evaluations were done. Based on the histopatological evaluation, no significant difference among each groups except the control group was noticed. In evoluation of neurological examination results, the treatment groups treated with E and NAS separately, it was observed that the neurologic examination performances at the 5th day has significantly rised according to trauma group. No significant difference was noticied by using the combination treatment. The results reveal that when applying E and NAS separately, the clinic neurological examination scores significantly had increased. It was pointed that functional and clinical improvements had been occured. In evaluation of biochemical results; when the control group and laminectomy group compared difference at the MDA (nmol/gr-tissue) values determined sicnificant statically ( p:0.016). We thought that, this difference have been occurs because of aedema after laminectomy. This aedema had been verified pathologically. When the control group is compared with the laser and the treatment group, no significant difference had been noticed. When the laser group was compared with the treatment groups, no significant difference had been noticed. These biochemical results reveal that E and NAS s treatment dose, applying time and rats sacrification time had been tought to be corallated. As a conclusion, the application of E and NAS separately in spinal cord injury clinical neurologic treatment had been observed. These treatments may tought to be used in the future after clinical investigations.
Key Words : 980- nm diode laser, spinal cord injury, Malondialdehide, Total antioxidant status, Nacetyl cisteine, Vitamin E.
v

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