ABSTRACT
THE EFFECT OF EXPERIMENTAL CONDITIONS ON LABELING YIELD OF 99mTc-ZOLEDRONIC ACID
Bone scintigraphy is a very sensitive method that is capable of planar and / or tomographic imaging. It has been being used since the discovery of the affinity of radioactive isotopes to bone . 99mTcBiphosphonate complexes are mostly preferred for bone scintigraphy. The most common used radiopharmaceutical for bone scintigraphy is 99mTc-MDP.
In this study, the aim is labeling Zoledronic Acid, a bone resorption inhibitor and a bisphosphonate derivative, with 99mTc. The effect of Zoledronic Acid, Stannous Chloride, Ascorbic Acid and 99mTc activity amount and pH value on labeling yield were investigated. In addition, bone imaging studies on rabbits were performed by gamma scintigraphy.
Zoledronic Acid was labeled with 99mTc using direct labeling method. The effect of experimental conditions on labeling efficiency, radiochemical purity and stability of the radiolabeled complex were determined by paper chromatography. The formulation which contains 0,05 mg Zoledronic Acid, 0,04 mg Stannous Chloride, 0,005 mg Ascorbic Acid and 3 mCi 99mTc was chosen as optimum formulation. It was observed that the labeling yield was greater than % 95 and the formulation was stable for at least 10 hours during the stability tests performed at room temperature (253 C).
In gamma scintigraphy studies, approximately 2,5 kg weighed, four female, New Zealand white rabbits were used. 0,75 mCi 99mTc-Zoledronic Acid was administrated intravenously to each rabbits. Static images at the times determined
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151. SAYFAYA BENZER SAYFALAR

tabs_sener_yagiz_191898 - Sayfa
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151. SAYFA ICERIGI

ABSTRACT
THE EFFECT OF EXPERIMENTAL CONDITIONS ON LABELING YIELD OF 99mTc-ZOLEDRONIC ACID
Bone scintigraphy is a very sensitive method that is capable of planar and / or tomographic imaging. It has been being used since the discovery of the affinity of radioactive isotopes to bone . 99mTcBiphosphonate complexes are mostly preferred for bone scintigraphy. The most common used radiopharmaceutical for bone scintigraphy is 99mTc-MDP.
In this study, the aim is labeling Zoledronic Acid, a bone resorption inhibitor and a bisphosphonate derivative, with 99mTc. The effect of Zoledronic Acid, Stannous Chloride, Ascorbic Acid and 99mTc activity amount and pH value on labeling yield were investigated. In addition, bone imaging studies on rabbits were performed by gamma scintigraphy.
Zoledronic Acid was labeled with 99mTc using direct labeling method. The effect of experimental conditions on labeling efficiency, radiochemical purity and stability of the radiolabeled complex were determined by paper chromatography. The formulation which contains 0,05 mg Zoledronic Acid, 0,04 mg Stannous Chloride, 0,005 mg Ascorbic Acid and 3 mCi 99mTc was chosen as optimum formulation. It was observed that the labeling yield was greater than % 95 and the formulation was stable for at least 10 hours during the stability tests performed at room temperature (253 C).
In gamma scintigraphy studies, approximately 2,5 kg weighed, four female, New Zealand white rabbits were used. 0,75 mCi 99mTc-Zoledronic Acid was administrated intravenously to each rabbits. Static images at the times determined
138




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